Leuconostoc oenos sp.nov.

Leuconostoc oenos sp.nov.

Nineteen strains of Gram-positive cocci isolated from wine and belonging to the genus Leuconostoc were examined and are considered to form a new species which is named Leuconostoc oenos. These strains differ from other leuconostocs because they grow better in a medium containing tomato juice than in yeast glucose citrate broth normally used in this laboratory for the genus. Furthermore, they gr...

Characterization of Leuconostoc oenos Isolated from Oregon Wines.

This study was designed to characterize isolates of Leuconostoc species from Oregon wines. Gram-positive cocci were isolated, and their biochemical properties and abilities to decompose malic acid were determined. All of the isolates were heterofermentative, catalase negative, and facultatively anaerobic and occurred in pairs and chains. They produced acid from glucose, fructose, mannose, ribos...

Biochemical basis for glucose-induced inhibition of malolactic fermentation in Leuconostoc oenos.

The sugar-induced inhibition of malolactic fermentation in cell suspensions of Leuconostoc oenos, recently reclassified as Oenococcus oeni (L. M. T. Dicks, F. Dellaglio, and M. D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995) was investigated by in vivo and in vitro nuclear magnetic resonance (NMR) spectroscopy and manometric techniques. At 2 mM, glucose inhibited malolactic fermentation ...

Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos.

Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension analysis showed that the mle operon has a single transcription start site located...

Citrate and Sugar Cofermentation in Leuconostoc oenos, a C Nuclear Magnetic Resonance Study